AC016405.3, a novel lncRNA, acts as a tumor suppressor via modulating of TET2 by miR-19a-5p sponging in glioblastoma.

Long non-coding RNAs (lncRNAs) are crucial regulators in various malignancies including glioblastoma multiforme (GBM). In the present study, we screened out a new lncRNA - AC016405.3 - through a previous genome-wide lncRNA microarray analysis in GBM. It showed that AC016405.3 was down-regulated in GBM tissue specimens and cell lines, and it also illustrated that the down-regulated AC016405.3 was closely correlated with several aggressive features of patients with GBM. Functionally, we displayed that overexpression of AC016405.3 suppressed GBM cells proliferation and metastasis via a gain of function experiment. We further showed that miR-19a-5p, a carcinogenic miRNA, was a downstream miRNA of AC016405.3. AC016405.3 was demonstrated as a target of miR-19a-5p, and overexpression of miR-19a-5p reversed the inhibitive effect of AC016405.3 on GBM cells proliferation and metastasis. Furthermore, a novel downstream gene of miR-19a-5p - ten-eleven-translocation-2 (TET2) was found through a constructed microarray analysis. We showed that TET2 was downregulated in GBM and was involved in miR-19a-5p mediated proliferation and metastasis by directly being targeted. Lastly, through a western blot assay and a series of functional cell counting kit 8 assays and metastatic assays, we displayed that AC016405.3 suppressed proliferation and metastasis through modulation of TET2 by sponging of miR-19a-5p in GBM cells. In summary, the findings of the current study found a novel lncRNA and illustrated that AC016405.3, acting as an anti-oncogene, suppressed GBM cells proliferation and metastasis by regulating TET via miR-19a-5p sponging. Our present study might provide a new axial in the molecular treatment of GBM. This article is protected by copyright. All rights reserved.