Changes in the profiling of bioactive components with the roasting process in Lycium chinense leaves and the anti-obesity effect of its bio-accessible fractions.

BACKGROUND: The present study aimed to investigate the profiles of bioactive components in roasted Lycium chinense leaves (LCL) and its in vitro anti-obesity activity after digestion processes.

RESULTS: Chlorogenic acid, kaempferol-3-sophoroside-7-glucoside, kaempferol-3-sophoroside, and kaempferol-3-glucoside were discovered as bioactive components in various ratios of EtOH extract in LCL by using ultra-performance liquid chromatography-electrospray ionization- mass spectrophotometry (UPLC-ESI-MS). The roasting process followed by a 30% EtOH extraction tended to decrease the content of chlorogenic acid and kaempferol-3-glucoside, and enhanced the content of kaempferol-3-sophoroside-7-glucoside. It effectively inhibited pancreatic lipase activity by 62.50±4.81%, which was approximately 1.71% higher than that of the dried-non roasted LCL extract (60.79±3.75%). Its bio-accessible fraction obtained from in vitro digestion significantly and dose-dependently reduced intracellular lipid accumulation by adipocyte 3T3-L1 compared to a 30% EtOH extraction. At the concentration of 200 μg mL-1 , it inhibited lipid accumulation up to 29.55 % in 3T3-L1 cells, which indicated that human digestive enzymes converted kaempferol-3-sophoroside-7-glucoside to kaempferol metabolites that have anti-obesity effects.

CONCLUSION: The current study suggests that the profiling of bioactive components by processing methods and a bio-accessible fraction could be crucial to improve the bioactivity of LCL, and potentially be a natural anti-obesity ingredient after oral intake. This article is protected by copyright. All rights reserved.