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Histochemical Staining of Cell Monolayers for β-Galactosidase.

Priti Kumar, Arvindhan Nagarajan, Pradeep D Uchil
Cold Spring Harbor Protocols 2019 March 2
There are several methods for assaying the success of transient transfections. If a plasmid expressing Escherichia coli β-galactosidase was used, then this histochemical staining procedure is simple to perform and yields dependable results. The following method was designed for cells growing in 60-mm culture dishes.

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