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A simple microfluidic assay for diagnosing tuberculous meningitis in HIV-uninfected patients.
Journal of Clinical Microbiology 2019 Februrary 28
We evaluated the diagnostic performance of a simple and label-free pathogen enrichment method using homobifunctional imidoesters and a microfluidic assay (SLIM) followed by real-time PCR from cerebrospinal fluid (CSF) in HIV-uninfected patients with suspected tuberculous meningitis (TBM). Patients with suspected TBM were prospectively enrolled in a tertiary hospital in an intermediate TB-burden country, during a 30-month period. TBM was classified according to the uniform case definition. Definite and probable TBM were regarded as the reference standards for TBM, and possible TBM and not-TBM as the reference standards for not-TBM. Of 72 HIV-uninfected patients with suspected TBM, 10 were diagnosed with definite (n=2) and probable (n=8) TBM by the uniform case definition. The sensitivity of the SLIM assay was 100% (95% CI, 69-100) compared with definite or probable TBM, and superior to mycobacterial culture (20% [95% CI, 3-56]) and Xpert MTB/RIF assay (0% [95% CI, 0-31]). Of 21 possible TBM and 41 not-TBM patients by the uniform case definition, 5 possible TBM and none of not-TBM gave positive results in the SLIM assay. The specificity of the SLIM assay was 92% (5/62, 95% CI, 82-97). We demonstrated that the SLIM assay had a very high sensitivity and specificity with small samples of 10 cases of definite or probable TBM. Further studies are needed to confirm this finding and to compare SLIM assay with mycobacterial culture, Xpert MTB/RIF, and Xpert MTB/RIF Ultra assay in a larger prospective cohort of patients with suspected TBM, including both HIV-infected and HIV-uninfected cases.
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