We have located links that may give you full text access.
Diagnosis of thalassemia using fluorescence spectroscopy, auto-analyzer, and hemoglobin electrophoresis - A prospective study.
Journal of Infection and Public Health 2019 Februrary 24
BACKGROUND: Hemoglobinopathies (HgP) are prevalent in certain regions of the world. The World Health Organization estimated that 5% of the world's population is a carrier of the potentially pathological hemoglobin (Hb) gene.
METHODS: This study aimed to compare the performance of fluorescence spectroscopy, a simple and inexpensive method, with that of conventional techniques for diagnosing thalassemia. The red blood cell (RBC) counts and levels of Hb, HbA, HbA2 , and HbS were estimated via conventional methods of complete blood count and Hb electrophoresis to diagnose thalassemia.
RESULTS: The RBCs and Hb, particularly the average values of HbA and HbA2 , were lower in patients with thalassemia than in the normal controls. These hematologic parameters were also analyzed via fluorescence spectroscopybased on fluorescent biomolecules including tyrosine (275 nm), tryptophan (290 nm), nicotinamide adenine dinucleotide (NADH) (370 nm), flavin adenine dinucleotide (FAD) (450 nm), and porphyrin (585-635 nm). In thalassemia patients, all these parameters were above the normal range, primarily due to abnormal depression of NADH and elevation of FAD.
CONCLUSION: Thalassemia canbe diagnosed via a fluorescent spectral method with an accuracy of 85% for blinded groups. This method may be useful for screening patients and reducing the cost of diagnosis in many rural countries.
METHODS: This study aimed to compare the performance of fluorescence spectroscopy, a simple and inexpensive method, with that of conventional techniques for diagnosing thalassemia. The red blood cell (RBC) counts and levels of Hb, HbA, HbA2 , and HbS were estimated via conventional methods of complete blood count and Hb electrophoresis to diagnose thalassemia.
RESULTS: The RBCs and Hb, particularly the average values of HbA and HbA2 , were lower in patients with thalassemia than in the normal controls. These hematologic parameters were also analyzed via fluorescence spectroscopybased on fluorescent biomolecules including tyrosine (275 nm), tryptophan (290 nm), nicotinamide adenine dinucleotide (NADH) (370 nm), flavin adenine dinucleotide (FAD) (450 nm), and porphyrin (585-635 nm). In thalassemia patients, all these parameters were above the normal range, primarily due to abnormal depression of NADH and elevation of FAD.
CONCLUSION: Thalassemia canbe diagnosed via a fluorescent spectral method with an accuracy of 85% for blinded groups. This method may be useful for screening patients and reducing the cost of diagnosis in many rural countries.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
A Guide to the Use of Vasopressors and Inotropes for Patients in Shock.Journal of Intensive Care Medicine 2024 April 14
Diagnosis and Management of Cardiac Sarcoidosis: A Scientific Statement From the American Heart Association.Circulation 2024 April 19
Essential thrombocythaemia: A contemporary approach with new drugs on the horizon.British Journal of Haematology 2024 April 9
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app