Biofabrication and characterization of flavonoid-loaded Ag, Au, Au-Ag bimetallic nanoparticles using seed extract of the plant Madhuca longifolia for the enhancement in wound healing bio-efficacy.

The present communication warrants the presence of significant wound healing bio-efficacy of aq. alc. extract of the seed (49.78%) of the plant Madhuca longifolia. A family of seven flavonoid fractions have been ascertained in the seed aq. alc. extract of the target plant using LCMS-8030 analysis. In vivo wound healing parameters (wound area, wound closure, epithelization period, skin breaking strength and hydroxyproline content) have been examined in Swiss albino mice models. Statistically significant (p < 0.001) enhancement in the wound healing bio-efficacy has been effectively induced using flavonoid-loaded gold: (Ml f@AuNps ), silver: (Ml f@AgNps ), and Au-Ag bimetallic: (Ml f@Au-AgNps ) nanoparticles. Among the biofabricated nano-biomaterials, Ml f@AgNps exhibited an exceptional enhancement in the wound healing bio-efficacy (80.33%) attaining almost to the level of reference drug Placentrex (84.02%). All the fabricated nano-biomaterials were thoroughly characterized using UV-Vis, XRD, FE-SEM, TEM, EDX, and DLS. The promising enhancement in the wound healing potential of the nano-biomaterial (Ml f@AgNps ) has been explained based on the cumulative effects of biological and nanotech parameters. The bio-fabricated (Ml f@AgNps ) nano-biomaterials using the plant M. longifolia have lustrous prospects for the development of complimentary herbal nanomedicine for scaling-up the wound healing bio-efficacy.