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Micro-RNA335-5p targeted inhibition of sKlotho and promoted oxidative stress-mediated aging of endothelial cells.

Biomarkers in Medicine 2019 Februrary 21
AIM: MiR-335-5p expression might induce endothelial cells (ECs) aging and target inhibit sKlotho. This study aimed to investigate whether oxidative stress evoked miR-335-5p expression and whether miR-335-5p-regulated ECs function through sKlotho.

METHODS: The expression of miR-335-5p was detected in human umbilical vein endothelial cells treated with H2 O2 . Subsequently, endothelial function and sKlotho expression were measured in human umbilical vein endothelial cells treated with H2 O2 and transfected with miR-335-5p mimics or inhibitor sequences. Vector containing reporting system of sKlotho3'- untranslated region with a miR-335-5p-binding site was constructed.

RESULTS: H2 O2 stimulation significantly increased miR-335-5p expression. Force overexpression miR-335-5p suppress ECs function and sKlotho expression. MiR-335-5p target regulated sKlotho.

CONCLUSION: MiR-335-5p might serve as a negative factor for endothelial homeostasis and a potential treatment target for atherosclerosis.

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