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Multi-templates based molecularly imprinted sodium alginate/MnO 2 for simultaneous enantiorecognition of lysine, alanine and cysteine isomers.
International Journal of Biological Macromolecules 2019 Februrary 17
Sodium alginate (SA) was electrodeposited onto the surface of a glassy carbon electrode (GCE), and then l-lysine (l-Lys), l-alanine (l-Ala) and l-cysteine (l-Cys) were simultaneously introduced to the surface of SA via electrostatic attractions. γ-MnO2 film was deposited potentiostatically onto the multi-templates immobilized SA from an aqueous mixture of K2 SO4 and MnSO4 . The templates of l-Lys, l-Ala and l-Cys were then oxidized and removed by cyclic voltammetry (CV) while maintaining the crystalline structures of SA and MnO2 . Finally, the molecularly imprinted SA/MnO2 was successfully applied in the simultaneous recognition of the isomers of Lys, Ala and Cys by differential pulse voltammetry (DPV). This work opens a new avenue in the construction of SA-based multi-templates molecularly imprinted systems for electrochemical simultaneous recognition of the isomers of several chiral amino acids.
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