Alteration of Crop Rotation in Continuous Pinellia ternate Cropping Soils Profiled via ITS Amplicon Sequencing.

Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Soil physicochemical properties and fungal diversity were significantly lower in continuous P. ternate cropping soil compared with those of uncultivated soil. In contrast, significant increases in soil factors and fungal diversity were detected after the continuous cropping soil was subjected to rotational cropping treatments, indicating a remediation effect of crop rotation on continuous cropping soil. Moreover, with the extension of wheat cropping in the rotations, fungal richness and most physicochemical properties exhibited a significantly increasing trend, suggesting that the remediation effect of crop rotation could be enhanced by extending the cropping of break crops. Furthermore, compared with those of continuous cropping soils, microbes that were potentially beneficial to P. ternate were enriched in the rhizosphere by rotational cropping, whereas microorganisms that supposedly lead to soil deterioration were suppressed. Redundancy analysis indicated that Podospora and Alternaria were the major microbes that were responsible for increasing soil physicochemical properties; by contrast, the deterioration in soil fertility was mainly attributed to Fusarium microorganisms. This article is protected by copyright. All rights reserved.