We have located links that may give you full text access.
Influence of culture conditions on the technological properties of Carnobacterium maltaromaticum CNCM I-3298 starters.
Journal of Applied Microbiology 2019 Februrary 15
AIM: Investigate the effect of a broad spectrum of culture conditions on the acidification activity and viability of Carnobacterium maltaromaticum CNCM I-3298, the main technological properties that determine the shelf-life of biological Time-Temperature Integrator labels.
METHODS AND RESULTS: Cells were cultivated at different temperatures (20 to 37 °C) and pH (6 to 9·5) according to a modified central composite design and harvested at increasing times up to 10 h of stationary phase. Acidification activity and viability of freeze-thawed concentrates were assessed in medium mimicking the biological label. Acidification activity was influenced by all three culture conditions, but pH and harvest time were the most influential. Viability was not significantly affected by the tested range of culture conditions.
CONCLUSIONS: C. maltaromaticum CNCM I-3298 must be cultivated at 20 °C, pH 6 and harvested at the beginning of stationary phase to exhibit fastest acidification activities. However, if slower acidification activities are pursued, the recommended culture conditions are 30 °C, pH 9·5 and a harvest time between 4 to 6 hours of stationary phase.
SIGNIFICANCE AND IMPACT OF THE STUDY: Quantifying the impact of fermentation temperature, pH and harvest time has led to a predictive model for the production of biological Time-Temperature Integrators covering a broad range of shelf-lives. This article is protected by copyright. All rights reserved.
METHODS AND RESULTS: Cells were cultivated at different temperatures (20 to 37 °C) and pH (6 to 9·5) according to a modified central composite design and harvested at increasing times up to 10 h of stationary phase. Acidification activity and viability of freeze-thawed concentrates were assessed in medium mimicking the biological label. Acidification activity was influenced by all three culture conditions, but pH and harvest time were the most influential. Viability was not significantly affected by the tested range of culture conditions.
CONCLUSIONS: C. maltaromaticum CNCM I-3298 must be cultivated at 20 °C, pH 6 and harvested at the beginning of stationary phase to exhibit fastest acidification activities. However, if slower acidification activities are pursued, the recommended culture conditions are 30 °C, pH 9·5 and a harvest time between 4 to 6 hours of stationary phase.
SIGNIFICANCE AND IMPACT OF THE STUDY: Quantifying the impact of fermentation temperature, pH and harvest time has led to a predictive model for the production of biological Time-Temperature Integrators covering a broad range of shelf-lives. This article is protected by copyright. All rights reserved.
Full text links
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app