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Inference of Gene Co-expression Networks from Single-Cell RNA-Sequencing Data.
Single-cell RNA-Sequencing is a pioneering extension of bulk-based RNA-Sequencing technology. The "guilt-by-association" heuristic has led to the use of gene co-expression networks to identify genes that are believed to be associated with a common cellular function. Many methods that were developed for bulk-based RNA-Sequencing data can continue to be applied to single-cell data, and several of the most widely used methods are explored. Several methods for leveraging the novel time information contained in single-cell data when constructing gene co-expression networks, which allows for the incorporation of directed associations, are also discussed.
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