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Depletion of cytotoxic (CD8+) T cells impairs implant fixation in rat cancellous bone.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society 2019 Februrary 10
As cytotoxic (CD8+ ) T cells seem to impair shaft fracture healing, we hypothesized that depletion of CD8+ cells would instead improve healing of cancellous bone. Additionally, we also tested if CD8-depletion would influence the healing of ruptured Achilles tendons. Rats received a single injection of either anti-CD8 antibodies or saline and put through surgery 24 hours later. 3 different surgical interventions were performed as follows: 1) a drill hole in the proximal tibia with microCT (BV/TV) to assess bone formation; 2) a screw in the proximal tibia with mechanical evaluation (pull-out force) to assess fracture healing; 3) Achilles tendon transection with mechanical evaluation (force-at-failure) to assess tendon healing. Furthermore, CD8-depletion was confirmed with flow cytometry on peripheral blood. Flow cytometric analysis confirmed depletion of CD8+ cells (p < 0.001). Contrary to our hypothesis, depletion of CD8+ cells reduced the implant pull-out force by 19% (p < 0.05) and stiffness by 34% (p < 0.01), although the bone formation in the drill holes was the same as in the controls. Tendon healing was unaffected by CD8-depletion. Our results suggest that CD8+ cells have an important part in cancellous bone healing.
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