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Quantification of punicalagins in commercial preparations and pomegranate cultivars, by liquid chromatography-mass spectrometry.

BACKGROUND: Pomegranate (Punica granatum L.) - a delicious fruit once used in ayurvedic medicine, now is largely known for the antioxidant properties of the juice, which has also been considered to have health benefits against diseases such as cancer and cardiovascular diseases. These beneficial effects are associated with the fruit's high content of polyphenolic compounds. High demand and lower production levels drive pomegranate prices up, which leads to the possibility of pomegranate products being adulterated, diluted or substituted. To ensure the presence of pomegranate in various preparations labelled as containing pomegranate, a simple method was developed to screen and quantify the specific punicalagins by mass spectrometry.

RESULTS: The present method was used to analyze several pure and mixed beverages from the U.S. market, and also to quantify the punicalagins in the juice of 14 pomegranate cultivars. Punicalagins were detected in all cultivars, with higher concentrations in whole fruit juices compared with aril juices. Amongst the 20 commercial beverages, punicalagins were not detected in 4 preparations.

CONCLUSION: The LC-MS method presented herein enables an easy and rapid quantification of the specific punicalagins. The latter was detected in all cultivar samples, thus confirming that punicalagin is a good marker to confirm the presence of any of these 14 pomegranate cultivars in commercial juices. The absence of the specific marker in four commercial preparations shows the necessity to have simple and rapid methods to confirm the presence of pomegranate in preparations. This article is protected by copyright. All rights reserved.

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