We have located links that may give you full text access.
Chemical and in vitro Toxicity Analysis of a Supercritical Fluid Extraction of Kava kava (Piper methysticum).
Journal of Ethnopharmacology 2019 January 31
ETHNOPHARMACOLOGICAL RELEVANCE: Kava and kava extracts have shown great potential as a way to minimize anxiety-associated symptoms and to help alleviate pain. Hepatoxicity has been associated with the consumption of kava products. The chemical compounds, kavalactones (KL) and flavokavains (FK) have been implicated in kava's psychotropic and possible hepatotoxic properties.
AIM OF THE STUDY: To investigate the kavalactone and flavokavain content and in vitro toxicity of KAVOA™ , a supercritical carbon dioxide extraction (SFE) of kava.
MATERIALS AND METHODS: Kavalactone and flavokavain content of SFE kava and noble kava root were determined following extraction in acetone, cell culture media, and water using ultra high-performance liquid chromatography (UHPLC). Using water extractions of the kava products, the cell viability and toxicity on the human hepatocellular carcinoma cell line (HepG2) were determined using luminescent and fluorescent assays, respectively. The half maximal inhibitory concentration (IC50 ) of the SFE kava and noble kava root, extracted in cell culture media, were determined utilizing a luminescent cell viability assay.
RESULTS: Quantification of the KAVOA™, a SFE extraction of kava and kava root showed similar profiles of kavalactone and flavokavain content. When normalized to total kavalactone content, the SFE kava showed a significant decrease in the content of Flavokavain B (FKB) when compared to the kava root. Water extracted SFE and root kava did not show a negative impact on cell viability and toxicity when compared to the vehicle control treated cells. IC50 values were determined for the SFE kava and kava root extracted in cell culture media in respect to cell viability, 78.63 and 47.65µg/mL, respectively.
CONCLUSIONS: KAVOA™, a supercritical carbon dioxide extraction of kava displays a similar kavalactone profile to a noble variety of kava. In relation to total kavalactone content, KAVOA™ also has a lower content of the cytotoxic compound FKB. Aqueous extractions of KAVOA™ and noble kava root had no significant negative impact on cell viability and toxicity on HepG2 cells when compared to vehicle controlled treated cells. Results indicate KAVOA™ demonstrates a similar in vitro safety profile to that of noble kava root when experiments are normalized to kavalactone content.
AIM OF THE STUDY: To investigate the kavalactone and flavokavain content and in vitro toxicity of KAVOA™ , a supercritical carbon dioxide extraction (SFE) of kava.
MATERIALS AND METHODS: Kavalactone and flavokavain content of SFE kava and noble kava root were determined following extraction in acetone, cell culture media, and water using ultra high-performance liquid chromatography (UHPLC). Using water extractions of the kava products, the cell viability and toxicity on the human hepatocellular carcinoma cell line (HepG2) were determined using luminescent and fluorescent assays, respectively. The half maximal inhibitory concentration (IC50 ) of the SFE kava and noble kava root, extracted in cell culture media, were determined utilizing a luminescent cell viability assay.
RESULTS: Quantification of the KAVOA™, a SFE extraction of kava and kava root showed similar profiles of kavalactone and flavokavain content. When normalized to total kavalactone content, the SFE kava showed a significant decrease in the content of Flavokavain B (FKB) when compared to the kava root. Water extracted SFE and root kava did not show a negative impact on cell viability and toxicity when compared to the vehicle control treated cells. IC50 values were determined for the SFE kava and kava root extracted in cell culture media in respect to cell viability, 78.63 and 47.65µg/mL, respectively.
CONCLUSIONS: KAVOA™, a supercritical carbon dioxide extraction of kava displays a similar kavalactone profile to a noble variety of kava. In relation to total kavalactone content, KAVOA™ also has a lower content of the cytotoxic compound FKB. Aqueous extractions of KAVOA™ and noble kava root had no significant negative impact on cell viability and toxicity on HepG2 cells when compared to vehicle controlled treated cells. Results indicate KAVOA™ demonstrates a similar in vitro safety profile to that of noble kava root when experiments are normalized to kavalactone content.
Full text links
Related Resources
Trending Papers
Heart failure with preserved ejection fraction: diagnosis, risk assessment, and treatment.Clinical Research in Cardiology : Official Journal of the German Cardiac Society 2024 April 12
Proximal versus distal diuretics in congestive heart failure.Nephrology, Dialysis, Transplantation 2024 Februrary 30
World Health Organization and International Consensus Classification of eosinophilic disorders: 2024 update on diagnosis, risk stratification, and management.American Journal of Hematology 2024 March 30
Efficacy and safety of pharmacotherapy in chronic insomnia: A review of clinical guidelines and case reports.Mental Health Clinician 2023 October
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app