Preparing low-copy number plasmid DNA using the ZymoPURE II Plasmid Maxiprep Kit.

Plasmids differ significantly in copy number due to their origin of replication, size, and associated insert. Several plasmids contain mutations that allow them to reach very high-copy numbers within the bacterial cell. However, depending on the objectives of the experiment or the nature of the cloned insert, it is not always possible to use high-copy number plasmid DNA.