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1 H- 13 C HMBC NMR experiments as a structural and analytical tool for the characterization of elusive trans/cis hydroperoxide isomers from oxidized unsaturated fatty acids in solution.

The radical-dependent oxidation of unsaturated fatty acids is a fundamental reaction in lipid chemistry, biochemistry and technology. We report herein the first successful application of 1 H-13 C HMBC NMR experiment for the identification and quantification of complex and minor (3.9 to 0.85 -per-cent) components of cis and trans primary hydroperoxide isomers of oxidized oleate and linoleate methyl esters in solution, without the need of laborious isolation of the individual components.

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