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Automated phase unwrapping in Doppler optical coherence tomography.
Journal of Biomedical Optics 2019 January
Phase wrapping is a crucial issue in Doppler optical coherence tomography (OCT) and restricts its automatic implementation for clinical applications that quantify total retinal blood flow. We propose an automated phase-unwrapping technique that takes advantage of the parabolic profile of blood flow velocity in vessels. Instead of inspecting the phase shift manually, the algorithm calculates the gradient magnitude of the phase shift on the cross-sectional image and automatically detects the presence of phase wrapping. The voxels affected by phase wrapping are corrected according to the determined flow direction adjacent to the vessel walls. We validated this technique in the rodent retina using a prototype visible-light OCT and in the human retina with a commercial infrared OCT system. We believe this signal processing method may well accelerate clinical applications of Doppler OCT in ophthalmology.
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