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Intracellular degradation of Helicobacter pylori VacA toxin as a determinant of gastric epithelial cell viability.
Infection and Immunity 2019 January 29
Helicobacter pylori VacA is a secreted pore-forming toxin that induces cell vacuolation in vitro and contributes to the pathogenesis of gastric cancer and peptic ulcer disease. We observed that purified VacA has relatively little effect on the viability of AGS gastric epithelial cells, but the presence of exogenous weak bases such as ammonium chloride (NH4 Cl) enhances the susceptibility of these cells to VacA-induced vacuolation and cell death. Therefore, we tested the hypothesis that NH4 Cl augments VacA toxicity by altering the intracellular trafficking of VacA or inhibiting intracellular VacA degradation. We observed VacA colocalization with LAMP1- and LC3-positive vesicles in both the presence and absence of NH4 Cl, indicating that NH4 Cl does not alter VacA trafficking to lysosomes or autophagosomes. Conversely, we found that supplemental NH4 Cl significantly increases the intracellular stability of VacA. By conducting experiments using chemical inhibitors, stable ATG5 knockdown cell lines, and ATG16L1 knockout cells (generated using CRISPR/Cas9), we show that VacA degradation is independent of autophagy and proteasome activity, but dependent on lysosomal acidification. We conclude that weak bases like ammonia, potentially generated during infection by H. pylori urease and other enzymes, enhance VacA toxicity by inhibiting toxin degradation.
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