We have located links that may give you full text access.
Metabolic Profiling of Dietary Polyphenols and Methylxanthines in Normal and Malignant Mammary Tissues from Breast Cancer Patients.
Molecular Nutrition & Food Research 2019 January 29
SCOPE: Dietary polyphenols may protect against breast cancer. However, it is unknown whether polyphenols reach human malignant breast tumours in molecular forms and(or) at concentrations likely to act against cancer.
METHODS & RESULTS: Breast cancer patients (n = 19) consumed three capsules daily from biopsy-confirmed diagnosis to surgery (6 ± 2 days). The capsules contained pomegranate, orange, lemon, olive, cocoa, and grape seed extracts plus resveratrol, providing 37 different phenolics (473.7 mg), theobromine and caffeine (19.7 mg). One hundred one metabolites were identified in urine, 69 in plasma, 39 in normal (NT) and 33 in malignant (MT) tissues by UPLC-ESI-QTOF-MS. Eight patients did not consume extracts and served as controls. Phenolic-derived metabolites in MT and NT were mainly glucuronidated and(or) sulphated. Some representative metabolites detected in MT (median and range, pmol g-1 ) were urolithin-A-3-O-glucuronide (26.2; 3.2-66.5), 2,5-dihydroxybenzoic acid (40.2; 27.7-52.2), resveratrol-3-O-sulphate (86.4; 7.8-224.4), dihydroresveratrol-3-O-glucuronide (109.9; 10.3-229.4), and theobromine (715.0; 153.9-3,216). Metabolites, as detected in breast tissues, did not exert antiproliferative or estrogenic/anti-estrogenic activities in MCF-7 breast cancer cells.
CONCLUSIONS: This is the first study that describes the metabolic profiling of dietary phenolics and methylxanthines in MT and NT comprehensively. Although phase-II conjugation might hamper a direct anti-cancer activity, long-term tumour-senescent chemoprevention cannot be discarded. This article is protected by copyright. All rights reserved.
METHODS & RESULTS: Breast cancer patients (n = 19) consumed three capsules daily from biopsy-confirmed diagnosis to surgery (6 ± 2 days). The capsules contained pomegranate, orange, lemon, olive, cocoa, and grape seed extracts plus resveratrol, providing 37 different phenolics (473.7 mg), theobromine and caffeine (19.7 mg). One hundred one metabolites were identified in urine, 69 in plasma, 39 in normal (NT) and 33 in malignant (MT) tissues by UPLC-ESI-QTOF-MS. Eight patients did not consume extracts and served as controls. Phenolic-derived metabolites in MT and NT were mainly glucuronidated and(or) sulphated. Some representative metabolites detected in MT (median and range, pmol g-1 ) were urolithin-A-3-O-glucuronide (26.2; 3.2-66.5), 2,5-dihydroxybenzoic acid (40.2; 27.7-52.2), resveratrol-3-O-sulphate (86.4; 7.8-224.4), dihydroresveratrol-3-O-glucuronide (109.9; 10.3-229.4), and theobromine (715.0; 153.9-3,216). Metabolites, as detected in breast tissues, did not exert antiproliferative or estrogenic/anti-estrogenic activities in MCF-7 breast cancer cells.
CONCLUSIONS: This is the first study that describes the metabolic profiling of dietary phenolics and methylxanthines in MT and NT comprehensively. Although phase-II conjugation might hamper a direct anti-cancer activity, long-term tumour-senescent chemoprevention cannot be discarded. This article is protected by copyright. All rights reserved.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app