Persistent systemic rotavirus vaccine infection in a child with X-linked SCID.

OBJECTIVE: The main aims of present study were to elucidate the systemic RVA infection and to clarify the genetic changes of persistent virus in the X-linked severe combined immunodeficiency (SCID) patient.

METHODS: RotaTeq vaccine (RV5) genotype specific real-time RT-PCR was used to monitor viral RNA load in serially collected serum and stool samples. Next-generation sequence analysis was used to determine genotype of the virus by sequencing 11 gene segments. Polyacrylamide gel electrophoresis (PAGE) analysis was used to identify rearrangement of viral genes. The gene rearrangement was examined in NSP5 gene by using Sanger sequence.

RESULTS: A seven-month-old boy demonstrated chronic diarrhea following the third administration of RV5 and failure to thrive. He was diagnosed with X-linked SCID and successfully underwent cord blood transplantation. High copy numbers of RV5 genotype G1 RNA were detected in serially collected stool and serum samples, and the kinetics of viral RNA loads were correlated with the degree of clinical disease. Next-generation sequence analysis revealed genetic reassortment at least between the strains WI79-9/G1P7[5] and WI79-4/G6P7[8] in the VP7 gene and the VP4 gene among the vaccine-derived rotavirus strains. Additionally, PAGE analysis suggested genetic rearrangements in several genes, and it was confirmed in the NSP5 gene by sequence analysis.

CONCLUSIONS: The kinetics of RVA RNA load in serum and stool samples was consistent with the clinical course of the patient. Among five genotypes of RV5 vaccine, G1 genotype replicated well in this patient. Reassortment and rearrangements were demonstrated in persistently infected G1 genotype of RV5. This article is protected by copyright. All rights reserved.