Draft genome sequence of a multidrug-resistant tetA/IncF-harbouring Escherichia coli ST906 obtained from a soil cultivated with jaboticaba (Plinia cauliflora).

OBJECTIVES: The aim of this study was to report the draft genome sequence of a multidrug-resistant Escherichia coli obtained from a jaboticaba (Plinia cauliflora) culture soil in Brazil.

METHODS: The whole genome was sequenced on an Illumina MiSeq Platform and was assembled using SPAdes v.3.9. All data analyses were performed using tools from the Center for Genomic Epidemiology and Geneious v.11.1.5 software. The genome was annotated using NCBI Prokaryotic Genome Annotation Pipeline v.3.2.

RESULTS: A total of 4,854 protein-coding sequences was presented with GC content of 50.4%. Resistome analysis showed acquired antimicrobial resistance genes to β-lactam (blaTEM-116 ), tetracycline [tetA and tet(34)], aminoglycoside (aadA1), trimethoprim (dfrA1) and macrolide (mphA). Mutations in Quinolone Resistance-Determining Regions (QRDR) of GyrA, ParC and ParE were detected. Three plasmid incompatibility groups were detected [IncFII (allele 24), IncFIB (allele 1) and ColRNAl]. In silico analysis showed the tetA and mphA genes are located inside the IncF plasmid. This isolate was classified as ST906 (singleton), serotype O156:H23-fimH61, commensal phylogroup B1 and presented virulence genes (iroN, ipfA, cma, gad, iss).

CONCLUSION: This is the first report of a draft genome sequence of an MDR E. coli ST906 serotype O156:H23-fimH61 obtained from a Brazilian soil. This draft genome sequence can be used to compare MDR E. coli isolated from different sources and also to better understand the spread of this clone worldwide.