We have located links that may give you full text access.
In vivo assessment of increased oxidation of branched-chain amino acids in glioblastoma.
Scientific Reports 2019 January 24
Altered branched-chain amino acids (BCAAs) metabolism is a distinctive feature of various cancers and plays an important role in sustaining tumor proliferation and aggressiveness. Despite the therapeutic and diagnostic potentials, the role of BCAA metabolism in cancer and the activities of associated enzymes remain unclear. Due to its pivotal role in BCAA metabolism and rapid cellular transport, hyperpolarized 13 C-labeled α-ketoisocaproate (KIC), the α-keto acid corresponding to leucine, can assess both BCAA aminotransferase (BCAT) and branched-chain α-keto acid dehydrogenase complex (BCKDC) activities via production of [1-13 C]leucine or 13 CO2 (and thus H13 CO3 - ), respectively. Here, we investigated BCAA metabolism of F98 rat glioma model in vivo using hyperpolarized 13 C-KIC. In tumor regions, we observed a decrease in 13 C-leucine production from injected hyperpolarized 13 C-KIC via BCAT compared to the contralateral normal-appearing brain, and an increase in H13 CO3 - , a catabolic product of KIC through the mitochondrial BCKDC. A parallel ex vivo 13 C NMR isotopomer analysis following steady-state infusion of [U-13 C]leucine to glioma-bearing rats verified the increased oxidation of leucine in glioma tissue. Both the in vivo hyperpolarized KIC imaging and the leucine infusion study indicate that KIC catabolism is upregulated through BCAT/BCKDC and further oxidized via the citric acid cycle in F98 glioma.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app