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A protocol for generating induced T cells by reprogramming B cells in vivo

Qitong Weng, Fangxiao Hu, Mengyun Zhang, Yong Dong, Cui Lv, Ying Wang, Xiaofei Liu, Jinyong Wang
Cell Regeneration 2018, 7 (1): 7-15
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo . This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.


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