Add like
Add dislike
Add to saved papers

Metabolic Engineering of Escherichia coli for para -Amino-Phenylethanol and para -Amino-Phenylacetic Acid Biosynthesis.

Aromatic amines are an important class of chemicals which are used as building blocks for the synthesis of polymers and pharmaceuticals. In this study we establish a de novo pathway for the biosynthesis of the aromatic amines para- amino-phenylethanol (PAPE) and para- amino-phenylacetic acid (4-APA) in Escherichia coli . We combined a synthetic para -amino-l-phenylalanine pathway with the fungal Ehrlich pathway. Therefore, we overexpressed the heterologous genes encoding 4-amino-4-deoxychorismate synthase ( pabAB from Corynebacterium glutamicum ), 4-amino-4-deoxychorismate mutase and 4-amino-4-deoxyprephenate dehydrogenase ( papB and papC from Streptomyces venezuelae ) and ThDP-dependent keto-acid decarboxylase ( aro 10 from Saccharomyces cerevisiae ) in E. coli . The resulting para -amino-phenylacetaldehyde either was reduced to PAPE or oxidized to 4-APA. The wild type strain E. coli LJ110 with a plasmid carrying these four genes produced (in shake flask cultures) 11 ± 1.5 mg l-1 of PAPE from glucose (4.5 g l-1 ). By the additional cloning and expression of feaB (phenylacetaldehyde dehydrogenase from E. coli ) 36 ± 5 mg l-1 of 4-APA were obtained from 4.5 g l-1 glucose. Competing reactions, such as the genes for aminotransferases ( aspC and tyrB ) or for biosynthesis of L-phenylalanine and L-tyrosine ( pheA, tyrA ) and for the regulator TyrR were removed. Additionally, the E. coli genes aroFBL were cloned and expressed from a second plasmid. The best producer strains of E. coli showed improved formation of PAPE and 4-APA, respectively. Plasmid-borne expression of an aldehyde reductase ( yahK from E. coli ) gave best values for PAPE production, whereas feaB -overexpression led to best values for 4-APA. In fed-batch cultivation, the best producer strains achieved 2.5 ± 0.15 g l-1 of PAPE from glucose (11% C mol mol-1 glucose) and 3.4 ± 0.3 g l-1 of 4-APA (17% C mol mol-1 glucose), respectively which are the highest values for recombinant strains reported so far.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app