Draft genome sequence of a metallo-β-lactamase (bla AIM-1 )-producing Klebsiella pneumoniae SM32 isolated from a patient with chronic diarrhea.

OBJECTIVES: Klebsiella pneumoniae colonized in human gastrointestinal tract is a significant risk factor for extra-intestinal infections in severely ill patients. Recent reports indicated the high rate of K. pneumoniae infections results from patients'own gut microbiota. Here we report the draft genome sequence of a multi-drug resistant (MDR) K.pneumoniae strain SM32 (ST1916) harbouring the blaAIM-1 gene isolated from a chronic diarrhoea patient in China.

METHODS: The whole genomic DNA was sequenced using Illumina MiSeq platform. The generated reads were de novo assembled using SOAPdenovo v2.04. All probable coding sequences were predicted by Glimmer v3.02 and annotated using information from GenBank, Pfam, COG and KEGG. Resistance-related genes was also further identified.

RESULTS: Klebsiella pneumoniae SM32 belongs to sequence type ST1916, and its draft genome was assembled into 165 contigs that comprised 5,238,542bp. A total of 5013 protein-coding sequences and several genes associated with resistance to β-lactams, colistin, tetracycline, aminoglycoside, fluoroquinolones and trimethoprim/sulfamethoxazole were preliminary identified.

CONCLUSIONS: Multidrug-resistant K. pneumoniae colonized in human gastrointestinal tract provides a potential reservoir for extra-intestinal infections. The genome sequence of K. pneumoniae SM32 will be helpful to reveal the key roles of mobile genetic elements in the adaptive translocation and spread of antibiotic resistance.