Dual-Signal Electrochemical Enantiospecific Recognition System via Competitive Supramolecular Host-Guest Interactions: The Case of Phenylalanine.

For developing highly selective and sensitive electrochemical sensors for chiral recognition, taking advantage of the synthetical properties of β-cyclodextrin (β-CD, strong host-guest recognition) and carbon nanotubes wrapped with reduced graphene oxide (CNTs@rGO, excellent electrochemical property and large surface area) as well as the differences in binding affinity between β-CD and guest molecules, a dual signal electrochemical sensing strategy was proposed herein for the first time in chiral recognition based on the competitive host-guest interaction between probe and chiral isomers with β-CD/CNTs@rGO. As a model system, rhodamine B (RhB) and phenylalanine enantiomers (D- and L-Phe) were introduced as probe and target enantiomers respectively. Due to the host-guest interaction, RhB can enter into the β-CD cavity, showing remarkable oxidation peak current of RhB. In the presence of L-Phe, competitive interaction with the β-CD cavity occurs and RhB are replaced by L-Phe owing to the stronger binding affinity between L-Phe and β-CD, which resulted that the peak current of RhB decreases and peak current of L-Phe appears, and interestingly, the changes of both signals linearly correlate with the concentration of L-Phe. As for D-Phe, it cannot replace RhB owing to the weaker binding affinity between D-Phe and β-CD. Based on this, a dual-signal electrochemical sensor was developed successfully for recognizing Phe. This dual-signal sensing strategy can provide highly selective and sensitive recognition compared to single-signal sensor and has important potential applications in chiral recognition.