MicroR-542-3p can mediate ILK and further inhibit cell proliferation, migration and invasion in osteosarcoma cells.

MiR-542-3p and its target gene integrin linked kinase ( ILK ) in human osteosarcoma together with the differentially expressed genes from osteosarcoma tissues was analyzed through bioinformatics analysis in this study. Real time quantitative polymerase chain reaction (qRT-PCR) and western blot showed that the miR-542-3p expression decreased while the ILK expression increased in the osteosarcoma tissues. The overexpressed miR-542-3p or silenced ILK restrained cell invasion, proliferation and migration and arrested cell cycle, facilitated cell apoptosis in U-2OS and 143B cells. The dual-luciferase assay confirmed the targeting relationship between miR-542-3p and ILK . MiR-542-3p overexpression inhibited osteosarcoma growth in vivo . In conclusion, miR-542-3p overexpression down-regulated its target gene ILK , promoted osteosarcoma cells apoptosis and inhibited their proliferation, migration and invasion.