Add like
Add dislike
Add to saved papers

Microwell scaffolds using collagen-IV and laminin-111 lead to improved insulin secretion of islets.

Intrahepatic islet-transplantation is a promising therapy for treatment of type 1 diabetes. During islet isolation, collagenase is used to extract islets from the pancreas, leading to loss of important cell-matrix interactions. Loss of the native pancreatic microenvironment is associated with apoptosis of islet cells, early graft failure, and poor islet function. The islet extracellular matrix is comprised of a specific combination of collagen, laminin, and fibronectin molecules. Reintroducing these molecules has been shown to boost the function, viability, morphology, and proliferation of β-cells. In this research, the effect of combinatorial ECM on islet function and survival was investigated. Specifically, thin film microwell array scaffolds made from two distinct biomaterials were coated with fibronectin, collagen type IV, laminin-111, laminin-332 or a combination thereof. We found that coatings containing a single type of ECM molecule, e.g. fibronectin or collagen, can improve short term islet function. However, these single proteins do not prevent loss of morphology and subsequent loss of islet function afterwards. In contrast, combining collagen-IV with laminin-111 at a ratio of 8:2 not only improved short term islet function, but also preserved islet structure and islet function on a longer term. This effect was reproducibly shown on poly(ester urethane) and poly(ethylene glycol terephthalate-b-butylene terephthalate) microwell islet delivery devices as well as tissue culture polystyrene. We concluded that bio-functionalization of inert biomaterials regardless of their molecular composition with a specific combination of islet ECM molecules can support and improve islet function over longer time-periods. Our data suggested that creating a biomimetic islet niche by bio-functionalization of biomaterials can significantly improve the endocrine function of β-cells. The creation of islet mimicking niches in islet delivery devices leads to an improvement of islet function by restoring part of the islet's extracellular matrix in these devices.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app