Effect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum .

Fusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks afterward F. nucleatum stimulated chicken. This study was to access the effectiveness of IgY on periodontitis and halitosis lured by F. nucleatum in vitro and in vivo . The growth of F. nucleatum was inhibited ( p <0.05) by different concentration IgY in vitro and the results of Halimeter shows volatile sulfur compounds (VSCs) were reduced to 904±57 ppb at concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short chain acids decrease 46.2% in 10mg/ml IgY, ammonia decrease 70% in 40mg/ml IgY, aldehydes and olefine ketones are almost unchanged. The ELISA assay revealed that IL-6 and TNF-α were decreased after 4 weeks' IgY treatment. Morphometric (X-Ray) and histological analyzes (HE) shows that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis.