Production of a novel N-terminal PEGylated crisantaspase.

Crisantaspase is an asparaginase enzyme produced by Erwinia chrysanthemi and used as to treat acute lymphoblastic leukemia (ALL) in case of hypersensivity to E. coli ASNase. The main disadvantages of crisantaspase are the short half-life (10 hours) and immunogenicity. In this sense, its PEGylated form (PEG-crisantaspase) could not only reduce immunogenicity but also improve plasma half-life. In this work, we developed a process to obtain a site-specific N-terminal PEGylated crisantaspase (PEG-crisantaspase). Crisantaspase was recombinantly expressed in E. coli BL21(DE3) strain cultivated in shaker and in a 2L bioreactor. Volumetric productivity in bioreactor increased 37% compared to shaker conditions (460 and 335 U L-1 h-1 respectively). Crisantaspase was extracted by osmotic shock and purified by cation exchange chromatography, presenting specific activity of 694 U mg-1, 21.7 purification fold and yield of 69%. Purified crisantaspase was PEGylated with 10 kDa mPEG-NHS at different pH values (6.5-9.0). The highest N-terminal pegylation yield (50%) was at pH 7.5 with the lowest poly-PEGylation ratio (7%). PEG-crisantaspase was purified by size exclusion chromatography and presented a kM value 3 times higher than crisantaspase (150 and 48.5 μM respectively). Nonetheless, PEG-crisantaspase was found to be more stable at high temperatures and over the time. In two weeks, crisantaspase lost 93% of its specific activity, while PEG-crisantaspase was stable for 20 days. Therefore, the novel PEG-crisantaspase enzyme represents a promising biobetter alternative for the treatment of ALL. This article is protected by copyright. All rights reserved.