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Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

Rong Zeng, Jinyan Luo, Shigang Gao, Lihui Xu, Zhiwei Song, Fuming Dai
Molecular and Cellular Probes 2018 December 22
Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves.

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