Green synthesis of silver-nanoparticles from Annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens.

Silver nanoparticles play a important role in controlling mosquito population as well as multi drug resistant pathogens without causing much harm to humans. In the present study was focused on green synthesis of silver nanoparticles against dengue causing vector ( Aedes aegypti ) and pathogens affecting humans. The synthesized silver nanoparticle was confirmed using UV- absorption spectrum range obtained at 416 nm, XRD, FTIR and HR-TEM analysis were used to determine the silver nanoparticle morphology and size with ∼6.48 ± 1.2-8.13 ± 0.18 nm and face centered cubic structure. The synthesized silver nanoparticles were exposed to fourth instar larvae of A. aegypti with different concentration (3-20 μg/mL) for 24 h and its elicit maximum mortality (100%) at their final concentration of 20 μg/mL and it's LC50 value was 4.43 μg/mL and LC90 value was 13.96 μg/mL, respectively. The minimum inhibitory activities of the tested pathogens were 125, 31.25, 62.5, 62.6 and 62.5 μg/mL for the Bacillus cereus, Staphylococcus aureus , Pseudomonas aeruginosa , Escherichia coli and Candida albicans respectively. Further, the synthesized silver nanoparticle shows a potent antimicrobial activity against all tested pathogens. Moreover the effect of silver nanoparticle against Red Blood Cells belonging to 'O' positive blood group were tested and does not cause higher hemolysis to the cells even at the highest concentration. Based on these finding, we strongly suggested that face centered cubic structured A. reticulata AgNPs is an eco-friendly and potent bio-medical agent and can be apply in wide range of application an alternative chemically synthesized metal nanoparticle.