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Bioprocessing and technoeconomic feasibility analysis of simultaneous production of d-psicose and ethanol using engineered yeast strain KAM-2GD.
Bioresource Technology 2018 December 12
The objective of this study was to analyze the processing and technoeconomic feasibility of coproduction of d-psicose and ethanol in a modified dry grind ethanol process. The yeast strain was constructed by expressing d-psicose 3-epimerases (DPE) in Sachharomyces cerevisiae. The strain was capable of converting d-fructose to d-psicose at 55 °C with a conversion efficiency of 26.6%. A comprehensive process model for modified dry grind ethanol plant with 396,000 MT/yr corn processing capacity was developed using SuperPro Designer. Predicted ethanol and d-psicose yields were 390.4 L and 75.3 kg per MT of corn, with total annual production of 154.6 million L and 29,835 MT respectively. The capital investment for the plant was estimated as 150.3 million USD with total operating cost of 85.2 million USD/yr. The unit production cost and minimum selling price of d-psicose with an internal rate of return of 15% were calculated as $0.43/kg and $1.29/kg respectively.
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