Anticancer activity modulation of an innovative solid formulation of extra virgin olive oil by cultured zeolite scaffolds.

This paper deals with the design and manufacture of pure and hybrid synthetic (Mixed Matrix Membranes, MMMs) zeolite scaffolds (containing various amount of zeolite crystals dispersed in a polymeric matrix) to obtain new biomaterials. These scaffolds can potentially be used in the field of translational medicine to obtain innovative results to address tumorigenesis mechanisms with the promotion of an effort to deal with technical methods and information. Since olive oil has beneficial effects in healthy human cells and slows down and/or inhibits cell growth, the aim of this work was to monitor the protective and beneficial antitumor effects of olive oil in a new solid formulation (Spread Bio-Oil) on cancer cell cultured on zeolite scaffolds. In order to investigate the cytotoxicity of the new bio-oil spread and to test antiproliferative activity on the cancer cells we used two phenotypically different human breast cancer cell lines (MCF-7 and MDA-MB-231) seeded on various morphologies of zeolite membranes. We report the fabrication and characterization of pure and hybrid (MMMs) zeolite membranes and evaluated the intensively cell adhesion, spreading and cell growth by adhesion test, MTT, optical microscopy analyses and Scanning Electronic Microscopy (SEM) microphotography analyses. Our results demonstrate that both cell lines adhered and grow on all zeolite surfaces and that both show better viability after Spread Bio-Oil treatments. All cell adhesions are a specific membrane-type and, in particular, MCF-7 cells interact and adhere preferentially on pure zeolite membranes. Cancer cells seem to recognize and prefer the characteristics of the supports according to the following trend: Co-ZSM-5 > Co-S-1 > 13X. Moreover, Co-ZSM-5 zeolite membranes were the best scaffolds and MDA-MB-231 cells after administration of Spread Bio-Oil showed less viability with respect to MCF-7 responding better to all concentrations of the innovative food. Our data indicate that Spread Bio-Oil decreases at very low concentration values (5, 10, 25, 50, 100, 200 and 300 μg/mL) cell proliferation in a dose- and time-dependent manner. The work confirms both the superiority of pure zeolite scaffolds for cultures of human normal and cancer cells and Spread Bio-Oil as an innovative food preserving all the beneficial and healthy properties of the extra virgin olive oil from which it derives.