Cold stress modulates redox signalling in murine fresh bone marrow cells and promotes osteoclast transformation.

CONTEXT: Alteration of redox signalling and RANK-L expression in FBMCs of mice exposed to different intensities of cold stress (15 °C, 8 °C and 4 °C) were studied.

OBJECTIVE: To understand the effects of varying intensities of cold stress on murine FBMCs and its impact on osteoclastogenesis.

MATERIALS AND METHODS: FBMCs were isolated from mice exposed to different intensities of cold stress and used for immunoblotting and biochemical assays. Bone histometry was also done.

RESULTS: Different intensities of cold stress perturb redox signalling in FBMCs and alters bone histometry. Higher RANK-L expressions were noted in FBMCs of mice exposed to 8 °C and 4 °C as compared with 15 °C.

DISCUSSION AND CONCLUSION: Cold stress boosts free radical production in FBMC's, which might enhance RANK-L expression, an indicator of osteoclastogenesis. Thus, we speculate that stronger cold stress (8 °C and 4 °C) contributes to the development of early bone loss.