We have located links that may give you full text access.
Breath water-based doubly labelled water method for the noninvasive determination of CO 2 production and energy expenditure in mice.
Isotopes in Environmental and Health Studies 2018 December
We explored a novel doubly labelled water (DLW) method based on breath water (BW-DLW) in mice to determine whole body CO2 production and energy expenditure noninvasively. The BW-DLW method was compared to the DLW based on blood plasma. Mice (n = 11, 43.5 ± 4.6 g body mass (BM)) were administered orally a single bolus of doubly labelled water (1.2 g H2 18 O kg BM-1 and 0.4 g 2 H2 O kg BM-1 , 99 atom% (AP) 18 O or 2 H). To sample breath water, the mice were placed into a respiration vessel. The exhaled water vapour was condensed in a cold-trap. The isotope enrichments of breath water were compared with plasma samples. The 2 H/1 H and 18 O/16 O isotope ratios were measured by means of isotope ratio mass spectrometry. The CO2 production (RCO2 ) was calculated from the 2 H and 18 O enrichments in breath water and plasma over 5 days. The isotope enrichments of breath water vs. plasma were correlated (R2 = 0.89 for 2 H and 0.95 for 18 O) linearly. The RCO2 determined based on breath water and plasma was not different (113.2 ± 12.7 vs. 111.4 ± 11.0 mmol d-1 ), respectively. In conclusion, the novel BW-DLW method is appropriate to obtain reliable estimates of RCO2 avoiding blood sampling.
Full text links
Trending Papers
A Personalized Approach to the Management of Congestion in Acute Heart Failure.Heart International 2023
Potential Mechanisms of the Protective Effects of the Cardiometabolic Drugs Type-2 Sodium-Glucose Transporter Inhibitors and Glucagon-like Peptide-1 Receptor Agonists in Heart Failure.International Journal of Molecular Sciences 2024 Februrary 21
The Effect of Albumin Administration in Critically Ill Patients: A Retrospective Single-Center Analysis.Critical Care Medicine 2024 Februrary 8
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app