Finding order in a bustling construction zone: quantitative imaging and analysis of cell wall assembly in plants.

Assembly of polysaccharide-based walls by plant cells involves the rapid synthesis, trafficking, and deposition of complex biopolymers, but how these events are controlled and coordinated to achieve a strong, resilient extracellular matrix has remained obscure for decades. Recent quantitative analyses of fluorescence microscopy data have revealed details of the trafficking and synthetic activity of cellulose synthases, and new methods for labeling matrix polymers have unveiled aspects of their regulated deposition in the wall. Detailed studies of the identity, architecture, activity, and trafficking of the proteins and protein complexes that synthesize wall polymers, combined with advances in image acquisition and analysis, will aid future efforts to dissect wall assembly.