Intraluminal Pressure Triggers Myogenic Response via Activation of Calcium Spark and Calcium-Activated Chloride Channel in Rat Renal Afferent Arteriole.

Myogenic contraction of renal arterioles is an important regulatory mechanism for renal blood flow autoregulation. We have previously demonstrated that integrin-mediated mechanical force increases the occurrence of Ca2+ sparks in freshly isolated renal vascular smooth muscle cells (VSMCs). To further test whether generation of Ca2+ sparks is a downstream signal of mechanotransduction in pressure induced myogenic constriction, the relationship between Ca2+ spark frequency and transmural perfusion pressure was investigated in intact VSMCs of pressurized rat afferent arterioles. Spontaneous Ca2+ sparks were found in VSMCs when afferent arterioles were perfused at 80 mmHg. The spark frequency was significantly increased when perfusion pressure was increased to 120 mmHg. Similar increase of spark frequency was also observed in arterioles stimulated with β1 -integrin activating antibody. Moreover, spark frequency was significantly higher in arterioles of spontaneous hypertensive rats (SHR) at 80 and 120 mmHg. Spontaneous membrane current recorded with whole-cell perforated-patch in renal VSMCs showed predominant activity of spontaneous transient inward currents (STICs) instead of spontaneous transient outward currents (STOCs) when holding potential was set close to physiological resting membrane potential. Real-time PCR and immunofluorescence confirmed the expression of Ca2+ -activated Cl- channel (ClCa ) TMEM16A in renal VSMCs. Inhibition of TMEM16A with T16Ainh-A01 impaired pressure-induced myogenic contraction in perfused afferent arterioles. Our study, hence, for the first time detected Ca2+ sparks in intact VSMCs of afferent arterioles and their frequencies were positively modulated by the perfusion pressure. Our results suggest that Ca2+ sparks may couple to ClCa channels and trigger pressure-induced myogenic constriction via membrane depolarization.