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Cytomegalovirus Infection: Mouse Model

Ilija Brizić, Berislav Lisnić, Wolfram Brune, Hartmut Hengel, Stipan Jonjić
Current Protocols in Immunology 2018 July 25, : e51
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc.


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