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Anti-biofilm activity against Gram-positive bacteria by biologically synthesized silver nanoparticles using Curcuma longa.

OBJECTIVE: To assess the biofilm inhibition activity of biosynthesized silver nanoparticles (AgNPs) using Curcuma longa.

METHODS: Characterizations of these biogenic nanoparticles were done by UV-Vis spectroscopy, TEM, EDX and nanoparticles tracking analyzer. Antibiofilm activity of AgNPs against S. aureus and S. pneumoniae was carried out by 96 well plate method. Further the combined effect of AgNPs with the antibiotic was studied against S. aureus and S. pneumoniae.

RESULTS: UV-spectroscopic analysis showed a surface plasmon resonance peak at 415nm. Shape of AgNPs is spherical with average size of 20-40 nm while the elemental silver was confirmed by EDX analysis. These biosynthesized AgNPs have anti-biofilm activity against S. aureus and S. pneumoniae. Furthermore, the combined effect of AgNPs with antibiotic, showed enhanced anti-biofilm activity against both the strains under study.

CONCLUSION: C.longa mediated AgNPs showed potent antibiofilm activity.

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