[Effects of aerobic exercise and dieting on chemerin and its receptor CMKLR1 in the livers of type 2 diabetic rats].

OBJECTIVE: To investigate the effects of aerobic exercise and dieting on chemerin and its receptor chemokine like receptor-1 (CMKLR1) in the livers of type 2 diabetes mellitus (DM) rats and its possible roles on the improvements of blood glucose and lipid metabolism.

METHODS: Fifty male SD rats were randomly divided into control group (Con, n =6) fed with normal diet and DM model group ( n =44) fed with high fat diet. After 8-week feeding, DM model rats were intraperitoneal injected streptozocin (STZ) at the dose of 30 mg/kg body weight to establish DM rats. The successfully established diabetes rats were randomly divided into 4 groups ( n =6):diabetes control group (DM), diabetes exercise group (EDM), diabetes dieting group by feeding normal diet (NDM) and diabetes exercise plus dieting group (ENDM). During the following 4 weeks, exercise rats participated in moderate intensity aerobic exercise on a treadmill with a progressing increasing load for 4 weeks. Fasting blood glucose (FBG) was determined by Roche glucometer. Serum levels of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein (LDL)) were detected by using full automatic biochemical analyzer. The mRNA and protein expressions of chemerin and CMKLR1 in the livers were detected by real time PCR and Western blot, respectively.

RESULTS: Accompanied with the increased serum levels of TC, TG, LDL and FBG in DM rats, the mRNA and protein levels of chemerinand CMKLR1 in the livers of DM rats were significant up-regulated. The serum levels of TC, TG, LDL and FBG were reduced in EDM, NDM and ENDM rats compared with those of DM rats, meanwhile,decreased protein levels of chemerin were found in the livers of EDM, NDM and ENDM rats with most significant in ENDM rats while increased protein levels of CMKLR1 in the livers of NDM and ENDM rats.

CONCLUSIONS: Four weeks of aerobic exercise with or without dieting decreased chemerin and increased CMKLR1 in the livers of DM rats at the protein levels, which might be attributed to the improvement of blood glucose and lipid metabolism of DM rats.