Read by QxMD icon Read

Abnormal expression of fibrosis markers, estrogen receptor α and stromal derived factor‑1/chemokine (C‑X‑C motif) receptor‑4 axis in intrauterine adhesions

Qin Zhou, Xixi Wu, Jianguo Hu, Rui Yuan
International Journal of Molecular Medicine 2018, 42 (1): 81-90
Intrauterine adhesions (IUAs) are mainly derived from fibrous tissue formation following endometrial damage. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)α and the stromal derived factor (SDF)‑1/C‑X‑C chemokine receptor type 4 (CXCR‑4) axis are abnormally expressed in IUA endometrium. A total of 76 human endometrial biopsy samples (normal, n=20; mild‑to‑moderate IUAs, n=40; and severe IUAs, n=16) were employed, and Sprague‑Dawley rat IUA models at different time points were constructed. Subsequently, the expression of transforming growth factor (TGF)‑β1, matrix metalloproteinase (MMP)‑9, ERα and the SDF‑1/CXCR‑4 axis was evaluated in human and rat IUAs using histology, immunohistochemistry, reverse transcription quantitative polymerase chain reaction and western blotting. In patients and rats with IUA formation, the expression of TGF‑β1, MMP‑9 and ERα was significantly higher compared with the control group at the mRNA and protein levels (P<0.05); in addition, in patients, the TGF‑β1, MMP‑9 and ERα levels were significantly higher in severe IUAs compared with those in mild‑to‑moderate IUA endometrium (P<0.05). Although the chemokine SDF‑1 level in rats increased significantly during the early postoperative phase (reaching a peak at the second estrus phase) in rat endometrium (P<0.05), its special receptor CXCR‑4 expression did not differ significantly compared with the control group in rats or patients (P>0.05). Our findings indicated that aberrant activation of fibrosis and expression of ERα may be involved in the pathology of IUA formation. The role of the SDF‑1/CXCR‑4 axis in IUAs as inflammatory medium in the short‑term or special homing factors for bone marrow mesenchymal stem cells requires further verification in in vivo animal models.


You need to log in or sign up for an account to be able to comment.

No comments yet, be the first to post one!

Related Papers

Available on the App Store

Available on the Play Store
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"