A Rhodamine B-based fluorescent probe for imaging Cu 2+ in maize roots

Ting Lv, Yongqian Xu, Hongjuan Li, Fengyu Liu, Shiguo Sun
Bioorganic & Medicinal Chemistry 2018 May 1, 26 (8): 1448-1452
A new Rhodamine B-based fluorescent probe (RBO) is successfully designed and synthesized, which is a higher selective and sensitive chemosensor for Cu2+ than other ions. Under physiological conditions (pH = 7.0), the non emission RBO displays a rapid fluorescence increase together with a color change after addition of Cu2+ and the detection limit is down to 28nM, which can clearly illustrate the distribution of Cu2+ with the help of laser scanning confocal microscope in plant tissues. Eventually, it confirmed that the Cu2+ accumulates mostly in the vascular cylinder and very less in the epidermal cells of maize roots, which is important to understand how the plants take up, transport and store in the Cu2+ .

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