Chitinophaga caseinilytica sp. nov., a casein hydrolysing bacterium isolated from forest soil

Ram Hari Dahal, Jaisoo Kim
Archives of Microbiology 2018, 200 (4): 645-651
A novel casein hydrolysing bacterium designated strain S-52T was isolated from Kyonggi University forest soil. Cells were strictly aerobic, Gram stain-negative, oxidase- and catalase- positive, non-motile, non-spore-forming, rod-shaped, and golden-yellow-pigmented. Strain S-52T hydrolysed casein. It was able to grow at 20-37 °C (optimum 25-32 °C), pH 6.5-11.0 (optimum 7-9.5), and at 3% (w/v) NaCl concentration. Strain exhibits flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain S-52T formed a lineage within the genus Chitinophaga that was distinct from other species of the genus. Closest member was Chitinophaga barathri YLT18T (97.16% 16 S rRNA gene sequence similarity). The major cellular fatty acids were iso-C15:0 , C16:1 ω5c, iso-C17:0 3-OH, and summed feature 3 (C16:1 ω7c and/or C16: 1 ω6c). MK-7 was sole respiratory quinone. The major polar lipid was phosphatidylethanolamine. The DNA G + C content of strain S-52T was 48.8 mol%. DNA-DNA relatedness of strain S-52T with Chitinophaga barathri was 42.5%. On the basis of phenotypic, genotypic, phylogenetic, and chemotaxonomic characterization, S-52T represents a novel species in the genus Chitinophaga, for which the name Chitinophaga caseinilytica sp. nov. is proposed. The type strain is S-52T (= KEMB 9005-540T  = KACC 19118T  = NBRC 112679T ).

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