JOURNAL ARTICLE

A rhodamine-triazole fluorescent chemodosimeter for Cu 2+ detection and its application in bioimaging

Kanokorn Wechakorn, Samran Prabpai, Kanoknetr Suksen, Phongthon Kanjanasirirat, Yongyut Pewkliang, Suparerk Borwornpinyo, Palangpon Kongsaeree
Luminescence: the Journal of Biological and Chemical Luminescence 2018, 33 (1): 64-70
29327432
A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy.

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