Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake.

Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T , were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37.8 and 33.7 mol%, respectively. DNA-DNA relatedness between strain IMCC26026T and F. myungsuense HMD1033T was 56.4 %, showing a novel species status of strain IMCC26026T . Major fatty acid constituents (>10 %) of strain IMCC26013T were iso-C15 : 1 G, C15 : 1 ω6c, C17 : 1 ω6c and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and those of strain IMCC26026T were iso-C15 : 0, anteiso-C15 : 0 and summed feature 3. Respiratory quinone detected in the two strains was MK-6. Both strains contained phosphatidylethanolamine as a major polar lipid. On the basis of these results, strains IMCC26013T and IMCC26026T were considered to represent novel species in the genus Flavobacterium, for which the names Flavobacterium chuncheonense (type strain IMCC26013T =KCTC 52573T =NBRC 112526T ), and Flavobacterium luteum (type strain IMCC26026T =KCTC 52572T =NBRC 112527T ) are proposed, respectively.