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Fluorescent and colorimetric detection of Fe(III) and Cu(II) by a difunctional rhodamine-based probe.
A new rhodamine B hydrazone derivative (probe L) was synthesized and characterized. The probe L had sufficiently satisfactory selective response to Fe3+ and Cu2+ ions among various interferential metal ions, and high sensitivity with the detection limit of 4.63×10-9 M and 5.264×10-7 M for Fe3+ and Cu2+ ions, respectively. In the presence of Fe3+ , the probe L exhibited turn-on orange fluorescence accompanied by color change from colorless to pink. Toward Cu2+ , the probe L showed significant color change from colorless to red purple. These remarkable orange fluorescence and color change made probe L suitable naked-eye identify for Fe3+ and Cu2+ ions. By means of Job's plot, Benesi-Hildebrand studies and FTIR spectra, both 1:1 binding modes (L-Fe3+ and L-Cu2+ ) were confirmed. The coordination mechanism and turn on/off fluorescence for L-Fe3+ and L-Cu2+ complexes were well explained by theoretical calculations. Moreover, probe L could be used as a quick, simple, visual test strip for Fe3+ and Cu2+ detection.
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