The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines

Mohsen Nikseresht, Ali Mohammad Kamali, Hamid Reza Rahimi, Hamdollah Delaviz, Mehdi Akbartabar Toori, Iraj Ragerdi Kashani, Reza Mahmoudi
Pharmacognosy Research 2017, 9 (1): 87-95

BACKGROUND: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear.

OBJECTIVE: This study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines.

MATERIALS AND METHODS: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC50levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner.

RESULTS: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells.

CONCLUSION: This study demonstrated an effective plant in preventing or treating breast cancer.

SUMMARY: Antioxidant compounds in Matricaria chamomilla have anticancer effects.Hydroalcoholic extract of M. chamomilla controls cellular proliferation and apoptosis induction.Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis.Hydroalcoholic extract of M. chamomilla prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner.M. chamomilla has low cytotoxic effects on natural cells. Abbreviations Used: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC50: 50% growth inhibition concentration; GSE: Grape seed extract.

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