JOURNAL ARTICLE

CD14(bright)CD16+ intermediate monocytes are induced by interleukin-10 and positively correlate with disease activity in rheumatoid arthritis

Masako Tsukamoto, Noriyuki Seta, Keiko Yoshimoto, Katsuya Suzuki, Kunihiro Yamaoka, Tsutomu Takeuchi
Arthritis Research & Therapy 2017 February 10, 19 (1): 28
28183329

BACKGROUND: Three different subsets of circulating human monocytes, CD14(bright)CD16- (classical), CD14(bright)CD16+ (intermediate), and CD14(dim)CD16+ (non-classical) have been recently identified. It has been reported that CD14(bright)CD16+ monocytes are increased in rheumatoid arthritis (RA). However, the role of each monocyte subset in the pathogenesis of RA is still unclear. The purpose of this study was to investigate the association of CD14(bright)CD16+ monocytes with RA.

METHODS: The study enrolled 35 patients with RA and 14 healthy volunteers. The three subsets of peripheral blood monocytes were analyzed by flow cytometry. Serum cytokines were measured at baseline in patients with RA and in healthy volunteers. CD14(bright)CD16- monocytes were isolated and cultured in vitro with different cytokines for 14 hours, and CD16 induction was assessed.

RESULTS: The proportion of CD14(bright)CD16+ monocytes, and serum interleukin (IL)-6, IL-8, and IL-10 were increased in patients with RA compared to healthy controls. The proportion of CD14brightCD16+ monocytes correlated with the disease activity of RA positively, whereas the proportion of CD14(bright)CD16- monocytes correlated negatively. When isolated CD14(bright)CD16- monocytes were stimulated with IL-6, IL-8, and IL-10, the only cytokine that significantly induced CD16 expression on the cells was IL-10.

CONCLUSIONS: The proportion of CD16(bright)CD14+ monocytes was positively correlated with RA disease activity. The expression of CD16 in monocytes was induced by IL-10 but not IL-6, and IL-8 was enhanced in the sera of patients with RA. Our results suggest that CD16(bright)CD14+ monocytes are involved in the pathogenesis of RA and that IL-10 is a key cytokine that regulates CD16 expression in monocytes.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
28183329
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"