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JOURNAL ARTICLE

A novel miniaturized biofilter based on silicon micropillars for nucleic acid extraction

Salvatore Petralia, Emanuele Luigi Sciuto, Sabrina Conoci
Analyst 2016 December 19, 142 (1): 140-146
27917431
New miniaturised microfluidic biofilter (BF) devices based on silicon micropillars have been developed and tested regarding their ability to extract HBV (Hepatitis B Virus) bacterial DNA from biological sample solutions. The device is composed of a silicon microchannel in which the pillars are distributed at the bottom surface. The extracted DNA solutions were analysed by real time PCR amplification and the biofilter performance was evaluated. The results obtained show that the DNA binding to the biofilters and the elution efficiency strictly depend on the pillars' geometrical dimensions and increase proportionally with the surface/volume ratio. The device exhibiting the best extraction efficiency was then tested in combination with a silicon integrated real time PCR amplification chip as a preliminary step towards the development of genetic point-of-care devices.

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